FVMPE-RS Multiphoton Laser Scanning Microscope

FVMPE-RS Multiphoton Laser Scanning Microscope

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Description

FEATURES

  • Designed for Deep Imaging
  • Capture Fast, Dynamic Cellular Processes
  • Spend Less Time Tuning the Laser
  • Optional Features for Advanced Applications
  • Intuitive Software Optimized for Multiphoton Observation
  • Choose From 3 Frames and 3 Laser Configurations
  • Modular Units Designed for Your Applications

FLUOVIEW FVMPE-RS

Laser Unit IR Pulsed Laser with Negative Chirp for Multiphoton Excitation <Spectra-Physics products>
MAITAI HPDS-OL: 690 nm — 1040 nm
MAITAI eHPDS-OL: 690 nm — 1040 nm
INSIGHT X3-OL: 680 nm — 1300 nm
INSIGHT X3 DUAL/DUALC-OL: 680 nm — 1300 nm + 1045 nm<Coherent products>
Chameleon Vision I Olympus : 690 nm – 1040 nm
Chameleon Vision II Olympus : 680 nm – 1080 nm
Chameleon Vision S Olympus : 690 nm – 1050 nm<Main IR pulsed laser>
One Laser System & Twin Lasers System: MAITAI HPDS-OL, MAITAI eHPDS-OL, INSIGHT X3-OL, Chameleon Vision I Olympus, Chameleon Vision II Olympus, Chameleon Vision S Olympus Dual Lines System:INSIGHT X3 DUAL-OL, INSIGHT X3 DUALC-OL

<Additional IR line/ Laser>
One Laser System: –
Dual Lines System: 1045 nm fixed line from INSIGHT X3 DUAL /DUALC-OL
Twin Lasers System: MAITAI HPDS-OL, MAITAI eHPDS-OL, INSIGHT X3-OL, Chameleon Vision I Olympus, Chameleon Vision II Olympus, Chameleon Vision S Olympus
Laser Combiner Introduction optic with AOM attenuation
(0 % – 100 %, 0.1 % increment)Including fully
automated beam expander,
XY shifter and two axes angle alignment.
(4 Axes Quadralign Auto Alignment optic)
Direct coupling to laser port of scanning unit.
<Dual Lines, Twin Lasers System>
Motorized light path switcher, with DM900, DM1000R, DM1100 to combine two IR wavelength
for imaging.
Single laser for visible light without AOTF [object Object]
Scanning and Detection Main Scanner Standard Laser Ports
  • 1
Detector Standard
  • Multi Alkali Photomultiplier 2 Channels
Cooled GaAsP-PMT 2 CH
  • Cooled GaAsP-PMT 2 channels plus Multi alkali photomultiplier 2 channels
Optional 4 CH
  • ✓
Photo Detection Method Analog Integration
  • ✓
Galvanometer Scanner (Normal Imaging) Galvanometer Mirror Scanner (X, Y)
  • Dual Scanner (Anti-Oxidization Silver Coating)
Scanning Modes 2D XY, XZ, XT, Xλ
3D XYZ, XYT, XYλ, XZT, XTλ, XZλ
4D XYZT, XZTλ, XYTλ
5D XYZTλ
Other ROI scanning: rectangle clip, ellipse, polygon, free area, line, free line and point
Scanning Speed
  • 512 x 512 with 1.1 s – 264 s
  • Pixel Time: 2 μs – 1000 μs
Scanning Zoom
  • 1.0 X – 50.0 X with 0.01 X increments
Resonant Scanner (High-Speed Imaging) Scanning Modes 2D XY, XZ, XT
3D XYZ, XYT, XZT
4D XYZT
5D –
other ROI scanning: rectangle clip, line
Scanning Speed 30 fps at 512 x 512, 438 fps at 512 x 32.
Scanning Zoom 1.0 X – 8.0 X with 0.01 X increment
Field Number (NA)
  • 18
Z-Drive
  • A motorized focus module inside the microscope is used
  • Minimum increment: 0.01 μm
Transmitted Light Detector Unit Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fi ber adaptation to microscope frame
Microscope Frame
  • Upright
  • Gantry
  • Inverted
System Control Controller
  • OS: Windows 7 Professional 64 bit
  • CPU: Intel Xeon 4 core 3.6 GHz
  • CPU: Intel Xeon E5-1620 (3.60 GHz) or higher
  • Memory: 12 GB
  • Hard Disk: 1 TB HDD x 2, 240 GB SSD
  • Graphics board: NVIDIA Quadro K600 1 GB
  • Display: 30 inch
  • Hardware sequencer for highly precise timing repeatability control
Power Supply Unit –
Optional Unit SIM Scanner
  • ✓
Software Basic Features Dark room matching GUI design. User-arrangeable layout.
Acquisition parameter reload features. Hard disk recording capability, adjust laser power and HV with Z-stack acquisition.
Z-stack with alpha blending, maximum-intensity projection, iso-surface rendering.
IR Laser Controlling
  • Fully Integrated IR Laser Wavelength Control
  • Deep Focus Mode
Optional Motorised-Stage Software XY motorised-stage control, map image acquisition for easy target locating. Tiling acquisition and software image stitching.
Define multiple areas for time-lapse imaging.
Optional Mapping and Multiplepoint Simulation Software Multiple-point stimulation and data acquisition software. Mapping multiple-point stimulation to generate reaction map. Filtering feature to select points.
Optional Sequencer Manager Advanced programmable software to define multiple imaging/ stimulation tasks and execute by hardware sequencer.
Minimum gap 100 ms delay between tasks.
Optional Auto Compensation Software Auto spherical aberration compensation software.
Control of objective lens with auto spherical aberration compensation function. Auto adjustment of motorized correction collar to find the best position at certain observation depth. Auto adjustment of correction collar along with Z movement.
Dimensions, Weight and Power Consumption Microscope with Scan Unit Dimensions (mm)
  • Vibration isolation table size:
  • One Laser and Dual Lines System: 1500 mm x 1650 mm (1500 mm x 1800 mm with Inverted Microscope System)
  • Twin Lasers System: 1500 mm x 2000 mm
Operating Environment (Indoor Use) Ambient Temperature Room temperature: 20 – 25 ℃
Maximum Relative Humidity 75 % or less at 25 °C requires continuous (24-hour) power supply

TruResolution Objectives

FV30-AC10SV FV30-AC25W
Magnifications 10 25
NA 0.6 1.05
W.D. (mm) 8 2
Cover Glass Thickness (mm) 0 – 0.23 0 – 0.23
Immersion Liquid SCALEVIEW-A2 (Water, Silicone oil, and Normal oil available) Water
Special features Auto Compensation, Optimized for multiphoton imaging Auto Compensation, Optimized for multiphoton imaging
Dimensions 56mm (W) x 106.5mm (D) x 95mm(H) 56mm (W) x 106.5mm (D) x 101mm(H)
Weight approx. 1kg approx. 1kg

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